Morphological and biochemical evaluation of the antifibrotic activity of the heather family berries extracts growing in Yugra region
https://doi.org/10.34680/2076-8052.2021.3(124).82-89
Abstract
The focus of the paper is to determine hepatoprotective properties of polyphenolic extracts of the fruits of cranberry (Vaccinium oxycoccus L.), blueberry (Vaccinium myrtillus L.), cranberry (Vaccinium vitis-idaea L.) and blueberry (Vaccinium uliginosum L.). The study was conducted on 70 male Wistar rats aged 7-8 months, weighing 210-230 g. Liver fibrosis was modeled by toxic damage with a solution of carbon tetrachloride (CCl4) – 0.2 ml/100g of body weight in refined olive oil (1:1). It has been proven that polyphenolic extracts of blueberry (Vaccinium myrtillus L.) (most pronounced), blueberry (Vaccinium uliginosum L.), cranberry (Vaccinium oxycoccus L.) and cranberry (Vaccinium vitis-idaea L.) (least pronounced) can reduce the severity of liver damage caused by CCl4. The obtained data suggest that the studied extracts can be used as correcting agents for fibrosis.
About the Authors
E. A. Krivykh
Ханты-Мансийская государственная медицинская академия
Russian Federation
Russian Federation
N. S. Kavushevskaya
Сургутский государственный университет
Russian Federation
Russian Federation
A. Е. Guliaev
National Laboratory Astana
Kazakhstan
Kazakhstan
L. V. Kovalenko
Сургутский государственный университет
Russian Federation
Russian Federation
References
1. Yoon S., Kang G., Eom G.H. HDAC Inhibitors: Therapeutic Potential in Fibrosis-Associated Human Diseases. Int J Mol Sci., 2019, no.20(6), p. E1329. DOI: 10.3390/ijms20061329.
2. Wynn, T.A. Cellular and molecular mechanisms of fibrosis. J. Pathol., 2008, vol.214, pp.199–210.
3. Wu D.D., Wang D.Y., Li H.M., et al. Hydrogen Sulfide as a Novel Regulatory Factor in Liver Health and Disease. Oxid Med Cell Longev. 2019 Jan 20;2019:3831713. DOI: 10.1155/2019/3831713
4. Ore A., Akinloye O.A. Oxidative Stress and Antioxidant Biomarkers in Clinical and Experimental Models of NonAlcoholic Fatty Liver Disease. Medicina (Kaunas), 2019, vol.55(2), p.E26. DOI: 10.3390/medicina55020026
5. Lin L., Zhou F., Shen S., et al. Fighting Liver Fibrosis with Naturally Occurring Antioxidants. Planta Med., 2018, vol.84(18), pp.1318-1333.
6. Kockerling D., Nathwani R., Forlano R., et al. Current and future pharmacological therapies for managing cirrhosis and its complications. World J Gastroenterol., 2019, vol.25(8), pp.888-908.
7. Ebrahimi H., Naderian M., Sohrabpour A.A. New Concepts on Reversibility and Targeting of Liver Fibrosis; A Review Article. Middle East J Dig Dis., 2018, vol.10(3), pp.133- 148.
8. Li S., Tan H.Y., Wang N., et al. The Potential and Action Mechanism of Polyphenols in the Treatment of Liver Diseases. Oxid Med Cell Longev., 2018, vol.2018, pp.18394818. DOI: 10.1155/2018/8394818
9. Sun J., Wu Y., Long C., et al. Anthocyanins isolated from blueberry ameliorates CCl4 induced liver fibrosis by modulation of oxidative stress, inflammation and stellate cell activation in mice. Food Chem Toxicol., 2018, vol.120, pp.491- 499.
10. Barthold S.W., Bayne K.A., Davis M.A. Guide for the care and use of laboratory animals. Washington: National Academy Press; 2011., Applied Research Ethics National Association (ARENA) and Office of Laboratory Animal Welfare (OLAW) Bethesda: National government publication; 2002. Institutional animal care and use committee guidebook.
11. Constandinou C., Henderson N., Iredale J. P. Modeling liver fibrosis in rodents. Methods in Molecular Medicine, 2005; vol.117, pp.237–250.
12. Yang Q., Xie R.J., Geng X.X. Effect of Danshao Huaxian capsule on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibrotic liver of rats. World J Gastroenterol, 2005, vol.11, pp.4953-4956.
13. Kim N.C., Graf T.N., Sparacino C.M., et al. Complete isolation and characterization of silybins and isosilybins from milk thistle (Silybum marianum). Org Biomol Chem., 2003,vol.1(10), pp.1684-9.
14. Loguercio C., Festi D. Silybin and the liver: From basic research to clinical practice. World J. Gastroenterol., 2011, vol.17, pp.2288–2301. Sokar S.S., El-Sayad M.E., Ghoneim M.E., et al. Combination of Sitagliptin and Silymarin ameliorates liver fibrosis induced by carbon tetrachloride in rats. Biomed Pharmacother., 2017, vol.89, pp.98-107.
15. Kheiripour N., Karimi J., Khodadadi I., et al. Hepatoprotective Effects of Silymarin on Liver Injury via Irisin Upregulation and Oxidative Stress Reduction in Rats with Type 2 Diabetes. Iran J Med Sci., 2019, vol.44(2), pp.108-117.
16. Cásedas G., Les F., Gómez-Serranillos M.P., et al. Anthocyanin profile, antioxidant activity and enzyme inhibiting properties of blueberry and cranberry juices: a comparative study. Food Funct., 2017, vol.8(11), pp.4187-4193.
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For citations:
Krivykh E.A., Kavushevskaya N.S., Guliaev A.Е., Kovalenko L.V. Morphological and biochemical evaluation of the antifibrotic activity of the heather family berries extracts growing in Yugra region. Title in english. 2021;(3(124)):82-89. (In Russ.) https://doi.org/10.34680/2076-8052.2021.3(124).82-89
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