Pathogenetic significance of surgical trauma in the development of morphological changes in the uterine horns and their correction

The analysis of the results of a morphological study carried out on 10 female dogs weighing 12.7-15.4 kg, depending on the regional ozone therapy after trauma to the visceral peritoneum of the uterine horns. The experimental studies were carried out in compliance with the principles of humane treatment of animals and in accordance with the ‘Rules for conducting studies using experimental animals’ of the Ministry of Health of the Russian Federation. In total, 2 series of experimental studies were carried out. In series I, regional ozone therapy was not performed in 5 animals after surgery and traumatization of the peritoneum of the fallopian tubes in the postoperative period. In series II, 5 animals intraoperatively after traumatizing the fallopian tubes were catheterized to the branch of the middle uterine artery, and in the postoperative period, ozonized saline was infused twice a day at the rate of 1.0 ml per 1 kg of animal weight for 5 days. After 14 days, a repeated laparotomy was performed, the injured uterine horn was excised for morphological examination. The preparations obtained during operations were fixed according to a standard technique, embedded in paraffin, ultrathin sections were prepared, and stained with hematoxylin with eosin and according to Van Gieson. The analysis criteria were changes in the morphological structure in comparison with the control data of the preparations obtained during the first operation. It was found that in series I, compared with indicators in II, where regional ozone therapy was carried out, there was a significant increase in connective tissue elements in the serous and underlying muscle layers (P <0.05), with a significant decrease in muscle fibers (P <0.05). The vascularization indices in series II significantly exceeded the results of the study in series I (P <0.05), where regional ozone therapy was not performed. Thus, carrying out regional ozone therapy in the early postoperative period creates the preconditions for reducing the risk of an increase in the specific gravity of connective tissue elements in the wall of the uterine horns, and, accordingly, reduces the risk of the influence of surgical trauma on the development of destructive morphological changes in them, which may result in reproductive changes associated with them violations. This research reveals the need for further investigation.

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