VIral RNA as a diagnostic marker of the severity of the clinical course of hepatitis A
https://doi.org/10.34680/2076-8052.2022.4(129).77-79
Abstract
Today, the main method to solve practical issues for the diagnosis and treatment of diseases is enzyme immunoassay. Molecular methods, primarily polymerase chain reaction combined with reverse transcription, make it possible to detect hepatitis A viral RNA (HAV RNA) in clinical samples (blood serum, saliva), determine the duration of virus replication, and confirm the diagnosis in complex diagnostic cases. The study found that in severe hepatitis A (GA), the RNA of the virus was determined in 100% of cases. Correlation analysis showed medium positive relationships between viral load and alanine aminotransferase activity (ALT), which were significant. In patients of the first group with severe GA, there was a statistically significant increase in ALT compared with the mild course. Thus, the determination of HAV RNA in the blood serum of patients with GA may have a diagnostic significance for the clinical course of the disease.
About the Authors
A. D. Ibrokhimova
Санкт-Петербургский государственный педиатрический медицинский университет, Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера
Russian Federation
Russian Federation
U. E. Kuznetsova
Санкт-Петербургский государственный педиатрический медицинский университет, Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера
Russian Federation
Russian Federation
References
1. Nikolenko O.Yu., et al. Sovremennyye metody laboratornoy diagnostiki virusnykh gepatitov [Modern methods of laboratory diagnosis of viral hepatitis]. Aktual'nyye problemy diagnostiki infektsionnykh zabolevaniy (mikrobiologiya, biotekhnologiya, epidemiologiya, parazitologiya): Sbornik nauchnoprakticheskikh rabot Mezhregional'noy nauch.-praktich. konf. [Actual problems of diagnosis of infectious diseases (microbiology, biotechnology, epidemiology, parasitology): Collection of scientific and practical works of the Interregional scientific and practical conference]. Ed. Kharseyeva G.G. Donetsk, 2015. Pp.113–117.
2. Onishchenko G.G., et al. Vodnaya vspyshka gepatita A v Nizhnem Novgorode [Water outbreak of hepatitis A in Nizhny Novgorod]. Epidemiologiya i infektsionnyye zabolevaniya — Epidemiology and Infectious Diseases, 2007, no.3, pp.4–9.
3. Bushmanova A.D., Novak K.E., Esaulenko E.V., Ostankova Yu.V., Danilova E.M. Molekulyarno-biologicheskiye metody diagnostiki gepatita A [Molecular biological methods for diagnosing hepatitis A]. Vestnik Novgorodskogo gosudarstvennogo universiteta — Vestnik NovSU. Issue: Medical Sciences, 2020, no.3(119), pp.32–38.
4. Sergeeva A.V. Virus gepatita A: osnovnyye kharakteristiki i laboratornaya diagnostika [Hepatitis A virus: main characteristics and laboratory diagnostics]. Spravochnik zaveduyushchego KDL — Handbook of the Head of the CDL, 2018, no.4, pp.29–32.
5. Vasneva Zh.P., Borisova Z.A., Bezkaravayny S.E. PTSR diagnostika virusnykh gepatitov [PCR diagnostics of viral hepatitis]. V sbornike: mezhdunarodnyye nauchnyye chteniya (pamyati G.A. Tikhova). Sbornik statey Mezhdunarodnoy nauchno-prakticheskoy konferentsii [In the collection: International Scientific Readings (in memory of G.A. Tikhov). Collection of articles of the International Scientific-Practical Conference]. Moscow, 2021, pp.34–35.
6. Ryaskova E.A., Lebedev M.Yu., Zimin Yu.V., Novikova N.A. Primeneniye polimeraznoy tsepnoy reaktsii v diagnostike virusnykh gepatitov [The use of polymerase chain reaction in the diagnosis of viral hepatitis]. Nizhegorodskiye vedomosti meditsiny — Nizhny Novgorod Bulletin of Medicine, 2008, no.8, pp.56–62.
7. Esaulenko E.V., Bushmanova A.D., Sukhoruk A.A. Kliniko-laboratornaya kharakteristika gepatita A u patsiyentov s markerami virusa gepatita V [Clinical and laboratory characteristics of hepatitis A in patients with hepatitis B virus markers]. Zhurnal infektologii — Journal Infectology, 2017, vol.9, no.3, pp.75–80.
8. Gorchakova O.V., Esaulenko E.V. Dlitel'nost' virusemii pri gepatite A [The duration of viremia in hepatitis A]. Vestnik Sankt-Peterburgskoy gosudarstvennoy meditsinskoy akademii im. I.I. Mechnikova — Herald of North-Western State Medical University named after I.I. Mechnikov, 2006, vol.7, no.4, pp.249–250.
9. Normann A., Jung C., Vallbracht A., Flehmig B. Time course of hepatitis A viremia and viral load in the blood of human hepatitis A patients. J. Med. Virol., 2004, vol.72(1), pp.10–16.
10. Kwon O.S., Byun K.S., Yeon J.E., Park S.H., Kim J.S., Kim J.H., Bak Y.T., Kim J.H., Lee C.H. Detection of hepatitis A viral RNA in sera of patients with acute hepatitis A. J. Gastroenterol. Hepatol., 2000, vol.15(9), pp.1043–1047.
11. De Paula V.S, Villar LM, Morais LM, Lewis-Ximenez LL, Niel C, Gaspar AM. Detection of hepatitis A virus RNA in serum during the window period of infection. J. Clin. Virol., 2004, vol.29(4), pp.254–259.
12. Fujiwara K., Yokosuka O. Diagnostic tests: Serum antihepatitis A virus antibody and hepatitis A virus RNA. Nihon Rinsho, 2005, 63, suppl. 7, pp.401–403.
13. Fujiwara K, Yokosuka O. Association between severity of hepatitis A and variations in hepatitis A viral RNA. Nihon Rinsho, 2004, 62, suppl.8, pp.458–461.
Review
For citations:
Ibrokhimova A.D., Kuznetsova U.E. VIral RNA as a diagnostic marker of the severity of the clinical course of hepatitis A. Title in english. 2022;(4(129)):77-79. (In Russ.) https://doi.org/10.34680/2076-8052.2022.4(129).77-79
Views:
47
Email this article 