vestnovsuВестник Новгородского государственного университетаVestnik of Novgorod State University2076-8052Новгородский государственный университет имени Ярослава Мудрого10.34680/2076-8052.2022.2(127).43-46vestnovsu-120Research ArticleПатологическая анатомияPathologic anatomyУльтрамикроскопические особенности строения С-клеток щитовидной железы крыс после 60-дневного воздействия бензоата натрияUltramicroscopic features of the structure of thyroid gland C cells in rats after 60 days of sodium benzoate exposureМорозовВ. Н.MorozovV. N.morozov_v@bsu.edu.ruМорозоваЕ. Н.MorozovaE. N.СолинА. В.SolinA. V.Белгородский государственный национальный исследовательский университетRussian Federation20222009202302(127)4346Copyright © Морозов В.Н., Морозова Е.Н., Солин А.В., 20232023Морозов В.Н., Морозова Е.Н., Солин А.В.Morozov V.N., Morozova E.N., Solin A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnovsu.elpub.ru/jour/article/view/120

Для достижения цели исследования было отобрано 12 белых половозрелых крыс-самцов, распределенных на две группы: 1-ю группу составили животные, получавшие ежедневно в течение 60 дней внутрижелудочно бензоат натрия в дозе 1000 мг/кг массы тела, а 2-ю группу — контрольные животные. Забор образцов щитовидной железы для электронномикроскопического исследования, проводку, изготовление срезов и их контрастирование выполняли по стандартному протоколу. У животных 1-й группы в цитоплазме С-клеток преобладали секреторные гранулы крупного размера, часть из них имели низкую электронную плотность, часть была с неоднородным содержимым, а другие остались электронно-плотными. Ядра парафолликулярных клеток имели узкий ободок гетерохроматина под кариолеммой. В большинстве митохондрий были видны кристы неправильной формы. Кроме того, присутствовало также локальное повреждение митохондрий. Обнаружены короткие и тонкие цистерны гранулярной эндоплазматической сети с небольшим количеством рибосом на их внешней поверхности. Вышеуказанные морфологические особенности парафолликулярных клеток щитовидной железы половозрелых крыс свидетельствуют об изменении механизмов синтеза кальцитонина в канальцах эндоплазматической сети, белковосинтетической функции клеток и ее энергообеспечения под воздействием бензоата натрия.

To achieve the aim of the study, 12 white mature male rats were selected and divided into two groups: the group I consisted of animals treated daily for 60 days intragastrically with sodium benzoate at a dose of 1000 mg/kg of body weight, and the group II are control animals. Samples of thyroid gland were taken for electron microscopy, processed, sectioned, and stained according to a standard protocol. In animals of the group I, large-sized secretory granules prevailed in the cytoplasm of C cells, some of them had low electron density, some had heterogeneous content, while others remained electron-dense. The nuclei of parafollicular cells had a narrow rim of heterochromatin under the karyolemma. Most of the mitochondria showed irregularly shaped cristae. In addition, local mitochondrial damage was also present. Short and thin cisterns of the rough endoplasmic reticulum with a small number of ribosomes on their outer surface were found. The above morphological features of the thyroid gland C cells in mature rats indicate a change in the mechanisms of calcitonin synthesis in the cisterns of the endoplasmic reticulum, the protein-synthetic function of cells and its energy supply under the influence of sodium benzoate.

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The authors declare that there are no conflicts of interest present.